Summary
Type: SPE
Application #: 304860
Catalog#: 730012 - SPE Cartridges, Chromabond, C18 ec, 3ml, PP, 200mg, 50/pk
Detection:
Conditions: Column conditioning: 1 mL methanol, then 1 mL 0.05 % formic acid
(Do not let run the column dry.)
Sample aspiration: prepared sample is passed through the column by vacuum
Washing: 1 mL 0.05 % formic acid, then 1 mL 45 % methanol
Elution: 1.5 mL methanol
Evaporation: nitrogen stream, 60 °C
Reconstitution: 100 µL of 20 % methanol + 0.01 % formic acid
Authors: Ye, L. et al.
Source: J. Chromatogr. B 860 (2007), 10–17